site stats

Biochemical characterization of rice glutelin

WebApr 1, 2002 · Very little is known about the gene that regulates the biochemical and cellular processes responsible for the formation of PBs. Rice mutants that show variations in the profile patterns of storage proteins have been identified and assigned into four classes (Kumamaru et al., 1988; Ogawa et al., 1989). WebJun 15, 2009 · In this study, transgenic a123 expressing glutelin with a mutation at the processing site proved to be useful for studying the glutelin sorting and assembly system in rice seeds. The results obtained using transgenic rice plants clearly address the functional role of post-translational processing from the glutelin precursor to the mature acidic ...

Biochemical characterization of rice glutelin. - Europe PMC

WebUnlike other cereals which accumulate prolamins as their primary nitrogen reserve, the major class of storage proteins in rice and oat are the glutelins and 12S globulins, respectively. These proteins are highly homologous at the primary sequence level to the leguminous US globulins. This similarity in protein structure extends to the molecular ... WebRice Glutelins SpringerLink Seed Proteins Cite as Rice Glutelins Fumio Takaiwa, Masahiro Ogawa & Thomas W. Okita Chapter Circuit is open Abstract Despite being the major … sometimes wmv youtube https://takedownfirearms.com

Biochemical and molecular characterization of a rice …

WebINTRODUCTION. The major endospermic rice protein is glutelin,[1, 2] which comprised up to 70 to 80% of total rice protein.Janick also found 70% of glutelin protein in rice flour as compared to other cereals, which were higher in prolamin contents. The biochemical characterization of rice proteins shows the important nutritional properties and these … WebSep 1, 2012 · The Osborne method comprising of a 2-step in vitro digestion of rice flour was used to extract albumin, globulin, prolamin, and glutelin by using pepsin and trypsin. Subsequently, the antioxidant … Expand WebRice glutelin cDNA clones isolated from a rice cDNA library constructed from immature endosperm encoded polypeptides containing the same number of amino acids including a 24 amino acid signal peptide, a 282 amino acid acidic subunit and a 193 amino acid basic subunit. SummaryRice glutelin cDNA clones were isolated from a rice cDNA library … sometimes with one i love walt whitman

Characterization of rice glutelin fibrils and their effect on

Category:Characterization and localization of rice (Oryza sativa L.) seed ...

Tags:Biochemical characterization of rice glutelin

Biochemical characterization of rice glutelin

Characterization and localization of rice (Oryza sativa L.) seed ...

WebJul 5, 2024 · Background Rice is not only an essential food but also a source of high quality protein. Polyploidy is an evolutionary trajectory in plants, and enhancing glutelin by polyploidization is an attractive strategy for improving the nutritional value of rice seeds and presents a great potential for enhancing the commercial value of rice. Elucidating the … WebThe rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis …

Biochemical characterization of rice glutelin

Did you know?

WebJun 1, 2003 · L. Q. Qu 81-298-38-8373 81-298-38-8397 [email protected] X. L. Wei H. Satoh T. Kumamaru M. Ogawa F. Takaiwa Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan … WebJun 1, 1985 · The two major subunits of rice glutelin, the acidic (alpha) and basic (beta) polypeptides were purified by chromatofocusing and cation …

WebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/α-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable … WebJan 1, 1992 · The 51-kDa protein is the rice glutelin precursor, which is processed to give rise to the 34-37-kDa u-subunit and 21-23-kDa subunit [3,7]. Similarly, the 14-kDa protein has been identified as a rice prolamine [7]. The 25- and 16-kDa proteins were the most abundant polypeptides in the globulin fraction (Fig. 1, lane C). ... Biochemical ...

WebMay 1, 1985 · The two major subunits of rice glutelin, the acidic (α) and basic (β) polypeptides were purified by chromatofocusing and cation exchange … WebNov 2, 2008 · A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin …

WebNov 2, 2008 · Rice glutelin originates from the same ancestral gene as 11S globulin. Rice glutelin is synthesized as a M r 57 000 (57 kDa) precursor protein and is transported …

WebThe rice (Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/α-2 encoded by glu4 while forming a new polypeptide of pI6.50/α-1. Experiments were conducted to identify the … sometimes wrong trainWebOct 1, 2024 · The charge density of individual rice glutelin and gum arabic solutions was determined as a function of pH (2.0–7.0) by measuring the zeta potential values in order to confirm the optimum pH for complex coacervation between rice glutelin and gum arabic (Fig. 1 B).The net charge of rice glutelin solutions at pH 3.0 is the most positive (7.2 ± … small computer desks for home targetWebREP-1 was further purified to a single polypeptide of 36 kDa. REP-1 digested in vitro both the acidic and basic subunits of rice glutelin, the major seed storage protein of rice. Determination of the N-terminal amino acid sequence and experiments with protease inhibitors indicated that REP-1 is a cysteine endopeptidase. small computer desks for home ukWebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable … small computer desks ikeaWebSep 1, 2024 · Here we investigated and characterized the formation of thermal-induced rice glutelin fibrils at 85 °C and pH 2.0. The potential effects of rice glutelin fibrils on the in vitro starch digestibility were also evaluated. Thioflavin T fluorescence spectral analysis revealed extensive formation of rice glutelin fibrils after 4 h of incubation at pH 2.0 and 85 °C. sometimes wont to dosometimes worn men\u0027sWebEurope PMC is an archive of life sciences journal literature. sometimes words can t express quotes