Flt3lg antibody flow cytometry
WebWe suggest K562 and HL-60 cells could be expressing Flt3 ligand/Flt3L. The antibody was raised against the extracellular domain of the protein, however please note … WebNov 15, 2024 · FLT3L hydrodynamic delivery and intraperitoneal injection. FLT3L was administered either by i.p. injection of 10 μg/mouse, or by hydrodynamic delivery (HDT) …
Flt3lg antibody flow cytometry
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WebApr 23, 2009 · Bars represent SD. § and ¶ refer to P values of comparison of the viable and apoptotic cell numbers in 1 μg FLT3LG antibody-treated MV4-11-R samples with those in isotype control. §§ and ¶¶ refer to P values of comparison of the viable and apoptotic cell numbers in 5 μg FLT3LG antibody-treated MV4-11-R samples with those in isotype ... WebFlt 3 ligand (Flt 3-L), variously designated Flt 3/Flk 2 ligand or FL, is a 17 kDa hematopoietic growth factor that stimulates the proliferation of stem and CD34+ progenitor cells and …
WebFlow cytometry is a lab test used to analyze characteristics of cells or particles. During the process, a sample of cells or particles is suspended in fluid and injected into a flow … WebPrepare desired antibody cocktail in Flow Cytometry Staining Buffer. Immediately prior to addition to cells, add FVD to antibody cocktail at 0.5–1 μL per sample to be stained. Add FVD/antibody cocktail to cell samples. Incubate for 30 minutes at 2–8°C; protect from light. Wash cells 1–2 times with Flow Cytometry Staining Buffer.
WebAug 23, 2024 · FLT3 ligand (FLT3LG) is predominantly produced by lymphocytes, especially T cells . Admittedly, FLT3LG has been shown to bind to FLT3 (receptor) on … WebFLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE\; MIM …
WebFigure 2. Antibody titration example.(A) Cells were incubated with serial dilutions of an APC-conjugated mouse anti-CD8 antibody, run on the Invitrogen Attune NxT Flow Cytometer, and analyzed using FlowJo software.Data are shown on a single graph for easier comparison. Note that the sample highlighted in box 1 is the saturation concentration of …
WebEdward A. Greenfield. If the antigen of interest is a cell-surface protein, flow cytometry or fluorescence-activated cell sorting (FACS) can be used to identify hybridomas secreting monoclonal ... old rich guyWebTechnological advancements in fluorescence flow cytometry and an ever‐expanding understanding of the complexity of the immune system have led to the development of large flow cytometry panels ... old rich ladyWebMar 21, 2024 · FLT3LG (Fms Related Receptor Tyrosine Kinase 3 Ligand) is a Protein Coding gene. Diseases associated with FLT3LG include Aplastic Anemia and Epiphyseal Dysplasia, Multiple, 3 . Among its related pathways are Insulin receptor signalling cascade and FLT3 signaling in disease . Gene Ontology (GO) annotations related to this gene … old rich familiesWebRecombinant Human FLT3LG Protein expressed from Escherichia coli (E. coli). ... Comparison List Basket Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: [email protected]. Home Antibodies ELISA Kits Primary Antibodies. Monoclonal Antibodies; ... Flow Cytometry Kits; Independently validated ELISA Kits; … old rich lady on couchWebAnti-FLT3LG Flow Cytometry Antibody Products Products (5) Write a Review Company View Product View Your search returned 5 FLT3LG Flow Cytometry Antibodies across … old rich girl namesWebMar 5, 2024 · Flow Cytometry Fluorescently labeled antibodies can be used to quantify cells of a specific type in a complex mixture using flow cytometry (Figure 20.5. 4 ), an … old rich homesWebIntracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Flt3 ligand/Flt3L with purified ab52648 at 1/50 dilution. Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). my office apps inc